• Centennial College

• BI 209

Experiment No: 6: Assay of antibiotic penicillin by observing zone of inhibition in

Staphylococcus aureus growth with the help of agar well diffusion method

Harita Khanvilkar

Student ID number: 300919468

Partner’s name: Aesha Patel

Course code: BI-209

Section number: 102

Date of submission: 17/08/2017

Instructor name: Mohamed I. Hasanar

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Antibiotic assay by agar diffusion method. BI-209-102

RESULTS

Initially four standard solutions of penicillin antibiotic were prepared of 0.64, 0.80, 1.25

and 1.56 units/mL concentration. All the standard penicillin solution and one unknown solution

were added in penicylinders in agar plate. The below given table shows the clear zone obtained

by inhibition of S.aureus growth in the presence of various concentration of penicillin. The agar

plates were incubated at 35° C for 48 hours.

Table No: 1: Zone of inhibition after inhibition of culture by various concentration of Penicillin.