Irvine High School

• BIO AP

• Fruit Fly Lab Report Instructions: In this virtual lab activity, you will investigate the preferences of fruit flies and compare their tactic responses to different environmental stimuli. After which, you will perform chi-square hypothesis testing to compare your data on fruit fly behavior. Submit your lab report to your instructor when completed. Please answer in PURPLE so that I can easily find your answers. Title: Effects of Fruit Fly Sex in Response to Stimuli Objective(s): ● observe preferences of the fruit fly; what they are attracted to ● responses to different environmental stimuli and how the sex of the fruit flies effects them ● compare results of the chi square hypothesis on fruit fly behavior Create your objectives based on the goals of the lesson and lab activity. Introduction–Fly Behavior in a Choice Chamber In this laboratory activity, you will observe the behavior of fruit flies in a choice chamber. Review a similar experiment scenario below and answer the pre-lab reflection questions. A student constructed a choice chamber by cutting off the bottoms of two plastic bottles (of the same size) and using clear plastic tape to tape the bottoms of the bottles together. She labeled one side A and one side B. She tapped 20 fruit flies into the choice chamber by placing a cap on one end and a funnel on the other end of the chamber. She tapped the flies into the chamber from a culture vial. After transfer, she replaced the cap and funnel with cotton balls. She placed five drops of distilled water on each of the two cotton balls and laid the chamber on a white piece of paper. After five minutes of undisturbed time, she counted the number of flies at each end of the choice chamber. The data she collected is below: Side A Side B 5 minutes 11 flies 8 flies 10 minutes 9 flies 12 flies Introduction–Fly Behavior Reflection Questions 1. Was there an overall orientation movement after 10 minutes? Explain the reason. a. No, there was not an overall orientation movement after 10 minutes since the numbers did not drastically change on the sides and only decreased and increased by a small amount of fruit flies on each side. 2. Describe the importance of using distilled water at both ends of the chamber? a. When using the distilled water at both ends of the chamber, it allows for the fruit flies to have a neutral group so there will be no bias in smell. That way the smell won’t impact