Stony Brook University BIO 204

3+4. Look in the lab 13 recitation slides for every answer! I didn’t know any of the answers
but I found it all in the recitation slides. Here’s screenshot of the answers
5. Agarose was the molecular sieve which basically means it provided the pores that acted as a
filter for the strands of DNA. Loading the dyes allowed us to predict migration as we could just
make out the colors of the strands before placing the gel in the UV light. The TAE buffer
contains EDTA which prevents the degradation of DNA by nucleases so it prevents the
degradation of DNA… it also provides the ions for current flow otherwise DNA wouldn’t move
and there wouldn’t be equal charge throughout the gel. The power supply provided the voltage.
The UV light allowed us to actually visualize the DNA fragments.
6a. So for part a the first thing is you see where the SNP is located (*) and insert a G there. It
then tells you that there will be two consecutive base pairs that need to be changed to make
the restriction site. The restriction site you want to make is either “CCTGCAGG” or
“GGACGTCC” if you look in the area on the gene around where the SNP is, you’ll see you
can make that sequence with AGTGCA*G. You make the * became a G, and the AG are the
two consecutive base pairs you need to mutate into a CC and that gives you the restriction
site CCTGCAGG
Answer: 5'-AGTGCAGG-3