Patrick Henry Community College

BIO 101

Begin by navigating your web browser to the following url: http://www1.udel.edu/biology/ketcham/microscope/scope.html Use the virtual compound microscope to practice finding the specimens on the slides and bringing them into focus. Use the checklist provided on the virtual lab to assure you are completing all the appropriate steps along the way. Answer the questions below on your worksheet. You should use a different font color or highlight your answers to make them easier for the instructor to see. Don’t forget to save your work before uploading your file and submitting your assignment. 1. Place the letter e slide in the microscope, focus, and center the object. What do you notice about the spatial orientation of the letter e? Its upside down and backwards 2. At the 10X objective, how many times its actual size does the letter e appear to your eye? (Don’t forget to account for the magnification of the objective and the ocular lenses). Once 3. Which objectives magnify the letter e to such an extent that the entire letter is no longer visible in the field of view (you may list more than 1: 4X, 10X, 40X, 100X)? 100X, 40X 4. Observe the onion root tip slide at the 4X objective. How did you adjust the iris diaphragm to produce the clearest image (lef = closed down, middle = half open, right = open)?